Federal State Budgetary Scientific Institution Research Articles

Liquid respiratory desaturation. The first experience of application on large biological objects

Introduction. This article is a continuation of the publication of the experimental studies results of prevention of acute decompression sickness (aDCS) in laboratory animals by the method of liquid respiratory desaturation (LRDS). LRDS is a method of preventing decompression disorders by the excretion of metabolically indifferent gases (in particular nitrogen) from the body during spontaneous respiration with liquid or artificial liquid lung ventilation (ALLV), by dissolved gas concentration gradient "from tissues to respiratory fluid". The method allows to provide rapid desaturation of the body from metabolically indifferent gases in the process of liquid respiration before and/or during decompression, thereby creating conditions for application of ultra-fast decompression profiles without the risk of decompression disorders.
 The aim of the study was to confirm the possibility of LRDS being a method of DCS prevention on large laboratory animals (minipigs).
 Materials and methods. The studies were performed on Wiessenau minipigs (n=8) — male and female, aged 4–5.5 months and weighing 7.1–11.2 kg. Animals of the control (n=4) and experimental (n=4) groups were subjected by compression by keeping in an air environment under high pressure (absolute 0.5 MPa for 60 minutes) to saturate tissues with indifferent gas (nitrogen). After compression experimental group was exposed to 30-minute ALLV with a liquid (perfluorodecalin) saturated with an oxygen under normobaric conditions. Air environment pressure during procedure of artificial lung ventilation was kept at 0.5 MPa. Decompression of the control and experimental groups was carried out in non-stop manner for 80 and 40 seconds respectively. The investigation of severity of clinical manifestations of DCS (changes in hemodynamic and respiratory parameters) including ultrasonic examination of gas bubbles in the heart and large vessels of the liver and as well as survival of animals in groups and pathoanatomical changes was performed.
 Results of the study. Clinical manifestations, ultrasonic scanning of the heart and venous vessels of the liver, as well as morphological examination data indicated the development of severe acute post-decompression disorders (PDD) in animals of the control group, which caused deaths in 100% of cases. Meanwhile all animals in experimental group survived and their state was stable. According to ultrasonic examination, the presence of small number of gas bubbles in the right-side heart chambers and liver's venous vessels being noted, but they disappeared after several hours. Deviations of the respiration's parameters function from background values (shortness of breath of a mixed type with the participation of auxiliary muscles, etc.) observed from the 2nd to the 4th day after ALLV, as well as compensatory reactions from the cardiovascular system (heart rate variability and instability of hemodynamic parameters) were caused by ALLV.
 Conclusions. Compressed air exposure 0.5 MPa for 60 minutes followed by 80 seconds of non-stop decompression allows to provoke the severe acute PDD minipigs model, manifested by pronounced intravascular gas formation, the development of acute respiratory and cardiovascular insufficiency causing the development of adverse outcomes.
 The ultrasound method of visual assessment of intravascular gas formation severity, adapted for minipigs, together with the dynamics of changes in the indicators of respiratory and cardiovascular systems makes possible to assess not only state of animal under anesthesia, but also the effectiveness of PDD prevention measures.
 The usage of ALLV with PFC liquid completely saturated with 100% oxygen under normobaric conditions makes possible partial removal of indifferent gas (nitrogen) dissolved in tissues of experimental animals during exposition in a compressed air environment before decompression and thereby carry out the prevention of DCS by LRDS method. It makes possible to implement successfully ultra-fast decompression profiles, incompatible with life in the control group.
 The state of animals from experimental group after LRDS is characterized by lung impairment caused by ALLV in hyperbaric conditions accompanied by temporary changes of external respiration function and compensatory reactions of cardiovascular system, observed during first 4 days.
 Ethics. The study was conducted in accordance with the ethical principles of the Declaration of Helsinki. The Clinical Study Protocol was approved by local Ethics Committee of The Federal State Budgetary Scientific Institution «Izmerov Research Institute of Occupational Health».

Tomato breeding for open ground in the south of Russia

   Relevance. Tomato is the most widespread vegetable crop in the southern regions of Russia and one of the most popular with the population in all natural and climatic zones of the country. The majority of products is produced in large vegetable-growing, farming and private household farms. One of the most important tasks is to improve the assortment taking into account natural and climatic conditions of cultivation and directions of use. In this regard, the selection of industrial tomato with a high level of flexibility and resistance to biotic and abiotic stressors for the south of Russia is an urgent task.   The aim of the study is to evaluate economically valuable features of perspective lines and new hybrids of tomato of industrial type, adapted to the conditions of southern Russia.   Material and methodology. The work was carried out in the open field conditions of experimental production farm of the Federal Scientific Center of Vegetable Industry (Moscow region) and Biryuchekutskaya vegetable breeding experimental station – branch of the FSBSI FSVC (Rostov region).   The objects of research were breeding material and 6 perspective new hybrid combinations of tomato of selection of the Federal State Budgetary Scientific and Scientific Institution of the Russian Federation.   Results and discussion. As a result of step-by-step study and selection, the most promising samples were identified for further use as source material for practical breeding: B-6-C-2014, VS-B-№7, VS-B-18, VS-B-20, VS-B-35, VS-B-38. As a result of the competitive testing, the best hybrid combinations were identified: F1 B-1, F1 B-2, F1 B-4, meeting the requirements of commercial production in the south of Russia. It is shown that the hybrid combination F1 B-2 exceeds both standard samples F1 Meteor and F1 Perfectpeel both in terms of total yield (by 10.9 t/ha and 9.6 t/ha), marketability of fruits, and dry matter content in fruits (6.1 % vs. 5.1% and 5.2 %). Taking into account the complex of economic characteristics, a precocious F1 hybrid of tomato F1 B-2 called F1 Profy has been submitted to the State Variety Testing, as it shows stable economic characteristics over several years of study.

The role of the Russian Academy of Sciences in the study of respiratory health and development of pulmonology in the Far East of Russia

The establishment of the Federal State Budgetary Scientific Institution «Far Eastern Scientific Center for Physiology and Pathology of Respiration» is examined within the historical progression of academic science in the Russian Far East and the Russian Academy of Sciences' contribution to this development, commemorating its 300th anniversary in Russia. The outcomes of medical science and healthcare contributions to pulmonology advancement in the Far Eastern region are evaluated.

Identification of the allelic state of the Rca2 gene for resistance to anthracnose and determination of genetic relatedness in garden strawberry genotypes

   The studies were conducted between 2022-2023.   The purpose of the work was to analyze the relationship of hybrids and varieties of strawberries resistant and susceptible to anthracnose and to determine the putative allelic status of the anthracnose resistance gene Rca2.   Selection of ISSR primers for identifying polymorphism and constructing dendrites of genetic similarity of 19 garden strawberry genotypes from the collection of the Federal State Budgetary Scientific Institution Federal Scientifi c Center for Horticulture. Th e eff ectiveness of ISSR primers was assessed by the ISSR-PCR method, followed by gel electrophoresis of ISSR-PCR products in an agarose gel. To assess the allelic state of the Rca2 gene, the SCAR marker STS-Rca2_240 was used. The genetic distance of the analyzed strawberry samples was determined using the Jaccard coeffi cient. Cluster analysis was carried out using the STATISTICA program (StatSoft ). The putative allelic status of the anthracnose resistance gene Rca2 in the studied varieties and hybrids was established. Diagnostic fragment of the STS-Rca2_240 marker, 240 bp in size. was found in the Alpha variety. Based on an array of genetic similarity coefficients, dendrograms were constructed, during the analysis of which the relationship of the studied variety samples was established. Promising forms were identifi ed that had a high degree of genetic similarity to varieties resistant to anthracnose, and forms that were closely related to susceptible varieties.

To the 75th Anniversary of Academician of the Russian Academy of Sciences Evgeny Lvovich Nasonov

On September 4, 2023, the 75th anniversary of the largest scientist, clinician and innovator, a talented teacher in the field of rheumatology, an outstanding healthcare organizer, scientific director of the Federal State Budgetary Scientific Institution “V.A. Nasonova Research Institute of Rheumatology”, Honored Scientist of the Russian Federation, Academician of the Russian Academy of Sciences Evgeny Lvovich Nasonov.

Cucumber breeding for spring film greenhouses for resistance to real powdery mildew

Relevance. Powdery mildew is one of the most common and harmful diseases of cucumber (Cucumis sativus L.) in open and protected ground. Moreover, in the closed ground, the harmfulness is higher than in the field. The yield of cucumber, due to the defeat of plants by powdery mildew, decreases by 30-50%, and in some cases by more than 70%. The creation of modern cucumber hybrids is impossible without the constant involvement in the breeding process of new sources of economically useful traits, including resistance to real powdery mildew. It is very important to combine in one line both a high degree of parthenocarpy, and the female type of flowering, and resistance to the most harmful diseases. We tried to solve this problem in our work.Materials and methods. The experience was laid in 2018-2022 in the Odintsovo district of the Moscow region on the basis of the head institution of the Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC) in the conditions of a spring ground greenhouse of the Block type, on a natural infectious background. On the basis of the laboratory of breeding and seed production of pumpkin crops, 27 collectible and about 50 breeding samples of parthenocarpic cucumber were studied. The article presents an assessment of the resistance to this powdery mildew of 36 of the most aligned and most valuable cucumber breeding samples in terms of a complex of economically useful features. 2-3 families, 6 plants in each, were evaluated for each sample for 2 years. During the entire growing season, the intensity of powdery mildew damage was assessed on a four-point scale and the spread of the disease in %.Results. Among the collection samples of cucumber, on a natural infectious background, two hybrids of the agrofirm BEJO ZADEN B.V. were not affected by powdery mildew: Amur 1801 F1, Artist F1, a hybrid without a name of the agrofirm MONSANTO HOLLAND B.V., conventionally named – Semenis F1 and hybrid Kibria F1 of the agrofirm RIJK ZWAAN. To a small extent, less than one point, they were amazed: Orpheus F1, Merengue F1, Marinda F1, Masha F1, Othello F1, List F1, Adam F1, SV 4097 F1 and Krasotka F1. 11cucumber breeding samples selected in previous years of research on a complex of economically useful signs that were not affected by this disease during 2 years of research were identified. Work will continue to evaluate these lines for resistance to real powdery mildew on an artificial infectious background.

A new functional food product – microgreen vegetable beans – feature and nutritional properties

Microgreens are valued for their freshness and refined taste, and by adherents of a healthy diet – for their saturation with vitamins, trace elements, antioxidants, enzymes and valuable protein. The product enjoys steadily growing demand not only in the production segment (restaurants, cafes, supermarkets), but also in home cultivation – «vegetable garden on the window». Among vegetable crops, vegetable pea micro-greens are very popular, especially varieties with a mustachioed leaf type. It is this product that helps culinary masters to give products bright visual and taste accents. Few people know that among legumes, in addition to vegetable peas, there is an excellent alternative with no less nutritional value. This is a micro-green of vegetable beans. Bean shoots are more fleshy and juicy, crispy, sweet with a nutty taste, and most importantly, absolutely everyone can grow them. Obtaining microgreens from vegetable bean culture is a promising direction, since its nutritional value and biochemical composition are comparable to the microgreens of vegetable peas. The content of the main nutrients on average for the studied varieties of breeding of the Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC) is: protein – 20-35% (dry weight), ascorbic acid – 0,51 mg/g, carotenoids – 0,48 mg/g, monosaccharide – 1,15%, dry matter – 10,62%. However, vegetable beans have an undeniable advantage in cultivation – it is the possibility of using up to two or three cuts from one crop, due to the ability to form additional shoots when using the method of cutting under the «root». The yield from the container when sowing 100 seeds obtained in two cuts ranged from 320 g to 400 g, depending on the variety and method of cutting. The highest yield was obtained from the cv. Velena, which forms about 200 g of fresh shoots both in the first and second cut. According to the results of our research, it is recommended to use two cuts for the cv. Belorusskie and cv. Russkie chernye, while the cv. Velena is able to give a full-fledged microgreen even with the third cut.

СОВРЕМЕННЫЕ ЗАДАЧИ НАУЧНЫХ ИССЛЕДОВАНИЙ В ОБЛАСТИ ВИНОДЕЛИЯ

The article presents the results of research activities for 2023 of the Federal State Budget Scientific Institution All-Russian National Research Institute of Viticulture and Winemaking Magarach of the RAS in the field of winemaking, carried out in accordance with the Program of Fundamental Scientific Research in the Russian Federation for the long term (2021-2030). The methodology of wine quality management with ecostatus has been created, aimed at revealing the individual characteristics of wines caused by natural factors and reducing the risks of negative effects on humans. Promising yeast strains have been identified: Steinberg, Champanskaya G-14, ShM-30, Novocimlyanskaya 3 with a description of their taxonomic affiliation, morphological and physiological-biochemical properties. Two strains of O. oeni active acid-reducing agents are recommended for practical use. The application of technology for the production of young sparkling wines of Crimean autochthonous grape The article presents the results of research activities for 2023 of the Federal State Budget Scientific Institution All-Russian National Research Institute of Viticulture and Winemaking Magarach of the RAS in the field of winemaking, carried out in accordance with the Program of Fundamental Scientific Research in the Russian Federation for the long term (2021-2030). The methodology of wine quality management with ecostatus has been created, aimed at revealing the individual characteristics of wines caused by natural factors and reducing the risks of negative effects on humans. Promising yeast strains have been identified: Steinberg, Champanskaya G-14, ShM-30, Novocimlyanskaya 3 with a description of their taxonomic affiliation, morphological and physiological-biochemical properties. Two strains of O. oeni active acid-reducing agents are recommended for practical use. The application of technology for the production of young sparkling wines of Crimean autochthonous grape varieties: Kokur belyi, Sary Pandas, Soldaiya and Kefesiya is justified. The system of criteria indicators for assessing the quality of grapes for the production of high-quality cognac distillates and cognacs is substantiated. A working collection of 63 natural strains of lactic acid bacteria has been formed. The optimal technological factors of the process of single-stage extraction by infusing with a food water-alcohol solution of green grape shoots of the Vitis vinifera, Vitis abrusca species have been established, allowing to increase the yield of total polyphenols by at least 2.6 times for the production of functional products. The most optimal technological modes of cold treatment of dry and fortified wine materials have been established to substantiate the constructive solution of an energy-saving installation for the purpose of cold treatment of wine materials based on the study of the main factors affecting the mass transfer process of potassium bitartrate crystallization. The obtained results of research work contribute to improving the quality of domestic wine products and expanding its production, including winemaking with ecological and geographical statuses, the production of domestic cognac and grape processing products of a functional orientation. The introduction of scientific developments into production will increase the competitiveness of domestic wine products, accelerate import substitution and ensure the country's food independence.

Влияние орошения на продуктивность отечественных сортов картофеля в условиях предкамья Республики Татарстан

The article presents the results of the study of varieties of Russian selecia presented by the Federal State Budgetary Scientific Institution "Federal Potato Research Center named after A.G. Lorch". In the studies conducted in 2023 on the basis of Agrobiotechnopark LLC at the Kazan State Agrarian University, the following potato varieties of domestic selection were presented: early-ripening - Meteor and Gulliver; medium-early potato varieties Ariel, Krasa Meschery and Surprise; medium-ripe - Grand and Pennant and Purple potato variety. Irrigation has had a positive effect on all the studied domestic potato varieties. When analyzing such indicators as the flatness of the development of the leaf surface, the height of the plant, the increase in the mass of the tops and tubers and taking into account the yield, all the studied varieties on irrigation gave the highest indicators than when cultivated on the bogar. The weather conditions of the growing season of 2023 differed significantly from the average long-term data. Since the air temperature in May, July and August was higher than the average annual values. As for, 46.79 mm of precipitation fell in May, which is 23 % higher than the average annual data, June was drier (only 6 mm of precipitation fell, which is 10% of the norm), in July and August precipitation fell only – 50.3 and 37.1 % of the monthly norm, respectively. According to the results of the analysis of the leaf surface area of domestic varieties, it can be judged that the use of irrigation on potatoes in 2023 showed positive dynamics in all variants compared to the cultivation of this crop on bogar. Thus, its maximum value of 19,909 thousand m2/ha in the flowering phase was achieved with irrigation of the medium-ripened potato variety Grand, which is 2205 thousand m2/ha higher than when cultivating this variety on bogar. The maximum yield of 21.8 t/ha was obtained for the medium-ripened potato variety Grand, which is 9.28 t/ ha higher when cultivating this variety without watering. The medium–early potato variety Ariel also formed a fairly high yield - 20.94 t/ ha.

Long-term consequences of wildfire smoke exposure on the central nervous system of rats in experimental modeling

Introduction. The annual increase in air temperature is one of the reasons for the occurrence of large-scale destructive fires throughout the world. The consequences of the negative impact of air pollution during forest fires on the respiratory and cardiovascular systems have been widely and well studied. At the same time, insufficient attention is paid to the influence of forest fire smoke on the functioning of the central nervous system.
 The study aims to study the morphofunctional state of the central nervous system of white male rats under prolonged exposure to forest fire smoke.
 Materials and methods. The study was conducted on 60 outbred white mature male rats weighing 200–220 grams. Animals in the experimental group (n=30) were exposed to daily smoke inhalation for 4 weeks, 4 hours a day, 5 days a week. Rats in the control group (n=30) were supplied with clean air into the chamber. The examination of animals exposed to smoke was carried out in two stages. At the first stage, the morphofunctional state of experimental animals was assessed 24 hours after the end of exposure. At the second stage — 60 days after exposure (long-term period). The examination included an analysis of indicators of the central nervous system: histology and morphometry of the brain.
 Results. Analysis of the results of the studied indicators indicates a decrease in motor and exploratory activity in animals exposed to smoke, as well as impairment of the ability to navigate and spatial memory. In the tissue structure of the sensorimotor cerebral cortex, multiple foci of neuronophagia, an increase in the number of degeneratively changed neurons, the formation of glial nodules and expansion of perivascular spaces were found. At the same time, the results of the examination in the long-term period indicated the absence of complete restoration of the identified violations.
 Conclusions. The results of experimental modeling revealed cause-and-effect relationships between exposure to forest fire smoke and disturbances in the morphofunctional state of the central nervous system.
 Limitations. The study is limited to studying the effects of prolonged 4 weeks, 5 days a week, 4 hours a day, exposure to wildfire smoke on outbred male white rats.
 Ethics. The study was conducted in compliance with the rules of humane treatment of animals in accordance with the Helsinki Association of the World Medical Association for the Humane Treatment of Animals (edition – October 2008) in accordance with the requirements of applicability to the protection of vertebrate animals or in another scientific research (ETS). No. 123), Directive 2010/63/EC of the European Parliament and of the Council of the European Union of September 22, 2010, on the protection of animals used for scientific purposes. Permission was obtained from the Local Ethics Committee of the Federal State Budgetary Scientific Institution VSIMEI to conduct experiments (protocol No. 32/19 dated May 10, 2019).

The study of the nutritional value of the national product «Kurt» produced at the enterprises of the East Kazakhstan region and the Abai region

The article presents the results of the physico-chemical parameters of the product "Kurt", produced by limited liability partnerships and individual entrepreneurs of the East Kazakhstan region and the Abai region.Kurt is a versatile fermented milk product that is added as a seasoning to various dishes and consumed as a separate food product. It is a good source of macro- and micronutrients in an easily digestible form for the body, contributing to the maintenance of beneficial intestinal microflora. The product is popular among children and adults and is in great demand as a healthy food product [1].In this regard, the study of the nutritional and biological value and quality of kurt is an urgent task.The purpose of the study is to study the nutritional value of the national fermented milk product "Kurt", produced at enterprises of the East Kazakhstan region and the Abai region.When determining the nutritional value of the national product "Kurt", physico-chemical parameters (mass fraction of protein, fat, ash, moisture, salt) were determined in 3 types of kurt of the most common enterprises of the East Kazakhstan region and the Abai region: Vostok-Moloko Corporation LLP, Bagration Ulan LLP, Individual Entrepreneur R.G.Petrosyan. The research was conducted at the V. M. Gorbatov Federal Scientific Center of the Food System of the Federal State Budgetary Scientific Institution of the Russian Academy of Sciences, Moscow.

Yield of spring durum wheat against the background of cellulolytic activity of the soil

Long-term studies were carried out in the field at the station on crop rotations and monocultures of the department of agriculture and resource-saving technologies of the Federal State Budgetary Scientific Institution "FNTs BST RAS", located in the Orenburg region (51.775125° N and 55.306547° E.). The purpose of the study was to establish the influence of the cellulolytic activity of the soil and the quantitative content of nitrate nitrogen in the soil in combination with meteorological factors on the yield of wheat grown for many years (21 years) in crop rotations and monoculture in the Southern Urals. The object of the study is spring durum wheat cultivated in crop rotations of various rotations, a set of crops and monoculture, as well as soil samples according to the experimental options. When determining the cellulolytic activity of the soil, the method of decomposition of linen fabric under the influence of microorganisms was used. The yield of durum wheat is influenced by such factors as predecessors, including their aftereffect in the crop rotation system, as well as the prolonged effect of mineral fertilizers. The average yield of durum wheat (for 21 years of research) in six-field crop rotations is 0.71 t/ha, with fertilizer 0.76 t/ha. Cultivation of durum wheat in two field crop rotation increases its yield by 0.07 t, in six fields 0.23 t/ha relative to monocultivation of the crop according to two backgrounds of soil nutrition. Cultivation of durum wheat in a six-field crop rotation on green manure fallow is accompanied by increased cellulolytic activity (12.0%). In the monoculture of durum wheat, the consumption of N-NO3 during the growing season increases to 20 mg with fertilizer up to 16 mg per kg of soil on an unfertilized background, which is associated with an increase in weediness and consumption of the macronutrient by the weed component. The share of output of grain units of durum wheat in six-field crop rotations is up to 16.3%, in two-field crop rotations up to 47.9%, with mono-cultivation of crops 100%.

Estimation of pea samples with different leaf types according to adaptability parameter

   The purpose of the current study was to identify productive collection samples with high adaptive properties for use in pea breeding.   The study was carried out at the Federal State Budgetary Scientific Institution “ARC “Donskoy”, located in the southern part of the Rostov region.   The objects of study were 100 collection pea samples from the collection of the ARIGRP named after N. I. Vavilov, 47 samples of which were with a usual (leafy) and 53 samples with a leafless type of leaf.   The variety ‘Aksaisky Usatiy 5’ was used as a standard. The years of study, differing in agroclimatic conditions during the vegetation period, made it possible to estimate collection samples according to adaptive properties. The mean productivity of the collection over the years of study was 2.10 t/ha (1.58–3.25 t/ha). Seed productivity in the group of samples with a leafless type (2.10 t/ha) was at the level with leafy samples (2.09 t/ha). At the same time, the coefficient of variation was lower in the group of leafless samples – 12.4 %, than in the group of leafy ones – 13.0 %. Seed productivity of the standard variety ‘Aksaisky Usatiy 5’ over the years of study was 2.00 t/ha, ranged from 1.42 to 3.30 t/ha. Among the leafy samples, the most productive ones were ‘OMK-3’ (2.46 t/ha), ‘221/73’ (2.55 t/ha), ‘269/80’ (2.67 t/ha), ‘525/80’ (2.68 t /ha), ‘Blagodatny’ (2.77 t/ha). Among the samples with a leafy type, the most productive ones turned out to be ‘Ramus’ (2.45 t/ha), ‘Svetozar’ (2.52 t/ha), ‘Lavr’ (2.55 t/ha), ‘Koral’ (2.56 t/ha), ‘Deviz’ (2.62 t/ha). The highest values of homeostaticity with high seed productivity were found in the samples with a usual leaf, such as ‘Blagodatny’ (4.14) and ‘525/80’ (5.23). And among the leafless samples, the best ones were ‘Deviz’ (7.57) and ‘Koral’ (7.60). The study results on the homeostaticity of seed productivity of pea samples were used in breeding work when selecting parental forms for hybridization.

Influence of mineral fertilizer on soil microflora

The biomass of organic matter entering the soil after a particular crop affects the formation of humus, the phytosanitary state of the soil, and agricultural crops can use nutrients from plant residues much more efficiently than from mineral fertilizers. The article presents the results of many years of experiments on the study of the effect of stubble and root residues on the biological activity of soddy-podzolic soil using the application method in a field six-field crop rotation in the conditions of the Republic of Mari El. The experimental part of the work was carried out in the field on the experimental field of the Mari Research Institute of Agriculture - a branch of the Federal State Budgetary Scientific Institution FARC of the North-East according to the experience laid down in 1998. The repetition of options is three times, the location of the plots is systematic. It has been established that the activity of soil microflora mainly depends on the presence of organic matter in the soil. The highest activity of cellulose-destroying microorganisms on a natural background of fertility is observed in the second crop rotation when manure is applied for potatoes - 22.9% in the first period of exposure and 54.7% in the second period of exposure. The lowest biological activity of the soil for the first 45 days was noted in the grain-grass crop rotation (83% of cereals) - 17.7% and 43.4% - in the second period of exposure. This is due to the deficiency of organic substances due to their insufficient intake. The application of mineral fertilizers at a dose of N60P60K60 for pre-sowing cultivation significantly increases the biological activity of the soil in relation to the unfertilized background, and a rather high intensity of decomposition of flax cloth was observed in the second crop rotation - for the first 45 days - 24.9%, for 90 days - 56.8%. Correlation analysis (1998-2019) between the average value of canvas decomposition under crops for the entire growing season and the value of the hydrothermal coefficient (HTC) showed a close direct relationship, which in the first exposure period (45 days) was 0.87-0.90, in the second period of exposure - 0.86...0.89.

ВЛИЯНИЕ БИО-ОРГАНО-МИНЕРАЛЬНОГО КОМПЛЕКСА АКМ НА БИОЛОГИЧЕСКУЮ АКТИВНОСТЬ ПОЧВЫ, ПРОДУКТИВНОСТЬ ЯБЛОНИ И КАЧЕСТВО ПЛОДОВ

Federal State Budgetary Scientific Institution «North Caucasian Research Institute of Mountain and Foothill Gardening, Nalchik, KBR, Russia in terms of nutrient content, which can be used in health conservation programs for the prevention, including endemic diseases of the population.

Bacterial Strains from the Bioresource Collection of the Federal State Budgetary Scientific Institution of the Federal Scientific Center for Biological Plant Protection, which Have Growth-Stimulating Activity against Winter Wheat Plants

Bacterial Strains from the Bioresource Collection of the Federal State Budgetary Scientific Institution of the Federal Scientific Center for Biological Plant Protection, which Have Growth-Stimulating Activity against Winter Wheat Plants

СЕЛЕКЦИЯ ТЕХНИЧЕСКОГО ВИНОГРАДА НА МОРОЗОУСТОЙЧИВОСТЬ И ЗИМОСТОЙКОСТЬ ВО ВСЕРОССИЙСКОМ НИИ ВИНОГРАДАРСТВА И ВИНОДЕЛИЯ ИМЕНИ Я.И. ПОТАПЕНКО

Breeding and varietal studies in All-Russian Research Ya.I. Potapenko Institute for Viticul-ture and Winemaking − branch of Federal State Budget Scientific Institution «Federal Rostov Agricultural Research Center» are carried out in the northernmost Russian region of industrial grapevine cultivation. New technical varieties have been bred that have a number of economi-cally valuable features inherent in wild Ameri-can species and Amur grapes, with berry quality close to European varieties. In recent years, technical varieties with white berry: Ledyanoy, Prestige, Izumrud, Nectariy and varieties with colored berry Lyrica, Krasnostop Carpi and Yakhontovy have been transferred to the State variety Testing. The results of the study of these grape varieties in the period from 2015 to 2022 are presented. These varieties have good winter hardiness, consistently high yields, re-sistance to major fungal diseases and are suita-ble for the production of high-quality dry wines. The article presents data on the main ag-robiological and technological indicators, me-chanical and chemical composition of bunches and data on winter hardiness. The tasting eval-uation of the wine material from the studied grape varieties is given. Based on the conduct-ed research, all tested varieties are recommend-ed for the production of high-quality dry wines. The studied grape varieties are used in further breeding work as sources of resistance to fungal diseases and adverse environmental conditions.

Оценка гибридных сеянцев яблони по основным хозяйственно-биологическим признакам в Оренбургской области

Abstract. The aim is to evaluate hybrid apple seedlings according to the main economic and biological characteristics in the conditions of the Orenburg region. Methods. The studies were carried out from 2002 to 2022 in the Orenburg branch of the Federal State Budget Scientific Institution of the Federal Scientific Center for Horticulture. The objects of research were 10 hybrid families of apple trees selected by the Federal State Budget Scientific Institution of the Federal Scientific Center for Horticulture. The main observations and records on the main economic and biological characteristics were carried out according to the methodology “Program and methodology for the selection of fruit, berry and nut crops”, “Program and methodology for the study of variety of fruit, berry and nut crops”, statistical processing of experimental data was carried out by the method of two-factor analysis of variance, where A is the variety, B is the year. The scientific novelty lies in the fact that as a result of the conducted research, promising hybrid forms with a complex of economically valuable traits, with a high potential for productivity and large-fruiting in the conditions of the Orenburg region have been identified. Results. The genetic collection of the Orenburg branch of the Federal State Budgetary Scientific Institution of the Federal Scientific Center for Horticulture serves as a fundamental basis for breeding work, which makes it possible to intensify the creation of adaptive, highly productive apple varieties. It has been established that the majority of hybrid apple seedlings have high winter hardiness. Weak freezing (0.2–0.5 points) characterizes hybrids of crossbreeding combinations, such as BratChud × Simvol, Kovrovoe × Anis Sverdlovskiy. Phytosanitary monitoring carried out on the site of hybrid apple seedlings showed that over the years of the study, weather conditions did not allow the development of diseases in the apple tree. On average, fruits in the offspring were 1-1, 1-5, 1-6, 1-7, 1-8, 1-9, 1-10 (140.0 ... 150.0 g). The greatest productivity was formed by hybrids of combinations of crossing BratChud × Simvol (24.6 kg/tree), Orskoe × Prizemlennoe (24.0 kg/tree) and Arkaim × Podarok Orenburzh’yu (24.2 kg/tree).

Селекция раннеспелых гибридов кукурузы универсального использования

Abstract. The expansion of the northern zone of corn-sowing in the Russian Federation, the increase in demand for corn hybrids capable of maturing in zones with a short frost-free period, forming a high yield of grain and green mass, adapted to various soil and climatic zones, requires intensification of breeding work for early maturity. The purpose of the research is the creation of a new early-ripening corn hybrid of universal use, characterized by adaptability to various soil and climatic conditions. The research was carried out at the Agricultural Research Center “Donskoy” and research institutions in Russia in 2018–2021. Methods. The main method of creating hybrids is the method of interline hybridization using heterosis in the first generation. Topcross crosses were used to obtain hybrid combinations. Object of the study are early ripe corn hybrids. A new early-ripening corn hybrid Vityaz’ MV of universal use was created jointly by the ARC “Donskoy” and the Federal State Budgetary Scientific Institution of the National Grain Center named after. P. P. Lukyanenko. It is included in the State Register of Breeding Achievements and approved for use in the production of the North Caucasian, Central, Central Black Earth and Middle Volga regions for grain and silage. According to the research results, the new hybrid is distinguished by high grain yield and plasticity. In the environmental test of research institutions in Russia, located in different soil and climatic conditions, the grain yield was 5.03–10.30 t/ha. In the ARC “Donskoy”, the average grain yield, including dry years, was 4.31 t/ha, which is significantly (by 0.36 t/ha) higher than that of the Krasnodarskiy 194 MV standard. The yield of green mass – 28.2 t/ha, dry matter – 9.41 t/ha. The new hybrid is drought-resistant, resistant to lodging and bunt smut, and is characterized by low harvest grain moisture content (14.0 %). The scientific novelty should be considered the creation of a new early-ripening corn hybrid Vityaz’ MV of universal use, adapted to various soil and climatic zones of the Russian Federation.

Особенности процесса ферментации молочно-соевой смеси

The plant milk market is actively developing at the moment, as many consumers suffer from lactose and casein intolerance, while vegetarianism is being popularized. The use of soy milk is becoming popular in the technology of fermented dairy plant products, as it is a lactose-free analog of cow milk and source of native protein, polyunsaturated fatty acids, vitamins, minerals, and other micronutrients. However, the fermentation of dairy and plant compositions is quite complex and has not been fully understood yet, and therefore the goal of this study was to conduct a comparative analysis of the fermentation of milk and soy mixtures with different compositions (cow milk: soy milk in the ratios of 55:45; 70:30; 85:15). The tests were conducted at the Laboratory of Agricultural Product Processing. To obtain milk and soy mixtures, a plant protein drink – soy milk – was prepared from soybeans of the Sentyabrinka variety selected by the Federal State Budgetary Scientific Institution Federal Research Center All-Russian Research Institute of Soybean. The fermentation of milk and soy mixtures was performed using the acid-rennet method with bacterial starter cultures No. 1 (Lactobacillus lactis, Lactococcus diacetylactis, Lactococcus cremoris, Streptococcus thermophilus) and No. 2 (Lactococcus lactis ssp. lactis, Lac. lactis subsp. diacetilactis, Leuconostoc lactis and/or mesenteroides subsp. cremoris, Lactobacillus plantarum, Lactobacillus helveticus, Streptococcus thermophilus). The acid formation of fermented milk and soy clots was studied in time at intervals of 1 hour, and their active and titratable acidity levels were analyzed in the course of work. Fermentation completion was determined based on the organoleptic indicators of fermented clots and their acidity levels. The following was established as the result of the tests: composition of the milk-soy mixture – 70:30; technological fermentation process parameters and modes – 328–346 minutes at a temperature of 34 °C, with the production of a fermented milk and soy clot having the best organoleptic quality indicators – well-formed, with a stable texture, pleasant, sour-milk taste, light soy aftertaste and smell. The analytical dependences of the titratable and active acidity indicators on the duration of fermentation process were calculated based on an analysis of acid formation in experimental clot samples.